MHH-ES-1
ACC 167
Cell line:
MHH-ES-1
DSMZ no.:
ACC 167
Species:
human (Homo sapiens)
Cell type:
Ewing's sarcoma
Origin:
established from the ascites of a 12-year-old boy with Ewing's sarcoma (type 2) of the left pelvis (with peritoneal metastasis); cells carry EWS-FLI1 fusion gene with t(11;22)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1.5-2 x 106 cells/80 cm2; split confluent culture 1:3 -1:5 every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca 48 hours
Harvest:
about 10-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 12% polyploidy - 49(46-51)<2n>XXY, +X, +1, +8, +12, -17, dup(1)(q31.1q32.2), del(3)(p11), der(4)t(4;?6)(p14;p11), t(6;7)(q11;p11), der(8)t(8;11)(p12;q13), t(11;22)(q24;q12) - presence of type-2 EWS-FLI1 hybrid transcripts (394 bp) confirmed by RT-PCR
Molec. Genetics:
expression of fusion gene EWS-FLI1 confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list