J-774A.1
ACC 170
Cell line:
J-774A.1
DSMZ no.:
ACC 170
Species:
murine (mouse) (Mus musculus)
Cell type:
monocytes-macrophages
Origin:
established from a tumor in a female BALB/c mouse in 1968; cells were described in the literature to synthesize lysozyme and interleukin-1 and to have receptors for immunoglobulin and complement
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 about twice a week (prior to reaching confluence); a certain amount of cells detach spontaneously if allowed to exceed confluence; adherent cells may be detached with a rubber policeman or using TEN (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl) (not trypsin/EDTA) for quantitative harvesting or for seeding out of cells collected from the supernatant for passaging; use always new culture flasks, otherwise cells are difficult to detach
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
about 4.5 x 107 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypotetraploid karyotype - 78(70-81)<4n> - in most cells a centric fragment was present - mouse chromosome morphology confirmed
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list