NRK-49F
ACC 172
Cell line:
NRK-49F
DSMZ no.:
ACC 172
Species:
rat (Rattus norvegicus)
Cell type:
kidney fibroblasts
Origin:
cell line was cloned from a mixed culture of normal rat kidney cells; cells exhibit contact inhibition and are very sensitive; indicator cells for viral or chemical transformation; this cell line is frequently used for TGF-ß bioassays
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
95% DMEM + 5% h.i. FBS
Subculture:
split culture 1:4 to 1:5 about 2-3 times a week using trypsin/EDTA when the cells have reached 3/4 of confluence; seed out at ca. 0.5-1.0 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of about 3.5 x 106/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
near-diploid rat karyotype with 4% polyploidy - 40(37-42)<2n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list