Leibniz Institute DSMZ: Details

Cell line:

IPL-LD-65Y

DSMZ no.:

ACC 181

Species:

insect - gypsy moth (Lymantria dispar) (Lymantria dispar)

Cell type:

larval cells

Origin:

established from the larvae of a gypsy moth (species Lymantria dispar, family Lymantriidae, order Lepidoptera) (Schwammspinner)

Reference(s):

not published

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

large cells; up to 30% grow adherent with processes; suspension cells are round to oval; image; image

Medium:

80% TC 100 medium + 20% h.i. FBS

Subculture:

cells are difficult to culture and grow slowly! split confluent culture 1:4 every 4-5 days using a (plastic) cell scraper

Incubation:

without CO₂ at room temperature (25-28 °C)

Harvest:

cell harvest of up to 10 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Species validation:

Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Lymantria dispar species.

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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