D-36
ACC 184
Cell line:
D-36
DSMZ no.:
ACC 184
Species:
murine (mouse) (Mus musculus)
Cell type:
mast cells
Origin:
established from the bone marrow of a DBA/2 mouse in 1986; cells have been described in the literature to be IL-10 sensitive
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Iscove's MDM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1 ng/ml mouse IL-4 + 1 ng/ml mouse IL-3
Subculture:
maintain at about 0.2-8.0 x 105 cells/ml; split ratio of 1:4 every 2-3 days; viability of cells may drop to about 20% immediately after thawing but recovers after approximately 1 week
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 25 hours
Harvest:
maximum density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype with 2% tetraploidy - 40(37-41)<2n> - centric fusion only rarely present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list