LN-405
ACC 189
Cell line:
LN-405
DSMZ no.:
ACC 189
Species:
human (Homo sapiens)
Cell type:
astrocytoma
Origin:
glioblastoma cells established from an astrocytoma tumor (grade IV) of a 62-year old woman in 1986; cells were described as expressing mRNA for both TGF-ß1 and G-TSF/TGF-ß2 (but only the latter polypeptide was secreted) and expressing GFAP protein
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:3 every 4-6 days using trypsin/EDTA; cells grow rather slowly; seed out at ca. 1.5 x 106 cells/80 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 60 hours
Harvest:
cell harvest of about 3-4 x 106 cells/175 cm2 or 1.5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertetraploid karyotype with 37% polyploidy and with 1-3 supernumerary dm present in most cells - 102-108<4n>XXXX, +1, +2, +3, +4, +5, +7, -10, +11, +13, -16, -16, -16, +19, +20, +20, +21, +22, +8mar, del(1)(q41)x2, der(1)t(1;3)(p32;p11), add(4)(p11), add(7)(p14), add(10)(p13), i(13q)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list