Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established from the peripheral blood of a male patient with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
Permissions and restrictions:
Morphology:
lymphoblastoid cells growing in clusters in suspension;
image;
image;
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Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.4-0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split saturated culture 1:3 every 3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
maximum density at about 1-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% tetraploidy - 47(44-48)<2n>X, +8, -13, t(2;?)(q35;?), t(8;13)(p12;q13), t(8;?)(p12;?)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
