L-929
ACC 2
Cell line:
L-929
DSMZ no.:
ACC 2
Species:
murine (mouse) (Mus musculus)
Cell type:
connective tissue fibroblasts
Origin:
established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 2.0-3.0 x 106 cells/80 cm2; split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2-3 days monolayer will be confluent; split 2-3 times a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-24 hours
Harvest:
ca. 15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype - 61-67, 12-16 centric fusion markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list