A-S-30D
ACC 208
Cell line:
A-S-30D
DSMZ no.:
ACC 208
Species:
rat (Rattus norvegicus)
Cell type:
hepatoma
Origin:
established from the hepatoma of a 16-month-old SD-female rat in 1988
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
optimal split ratio of 1:2 to 1:3 twice per week
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 29 hours
Harvest:
cell harvest of about 0.6 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
hypodiploid rat karyotype with 12% tetraploidy - 38(35-41)<2n> - 2 large submetacentric and 1 large metacentric centric fusion markers - sideline with 30-32/60-62 present at 14%
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list