NUC-1
ACC 225
Cell line:
NUC-1
DSMZ no.:
ACC 225
Species:
murine (mouse) (Mus musculus)
Cell type:
urinary bladder
Origin:
established from an adult female C3H/Law mouse; tumorigenic, invasive in vitro immortalized urine bladder cell line, aneuploid (chromosome number 70-75)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
45% Ham's F10 + 45% DMEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 µM HEPES
Subculture:
split confluent culture 1:10 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24 hours
Harvest:
harvest of about 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
hypotetraploid flat-moded murine karyotype with limited centric fusion, dm present in 25% cells - 70(66-75)<4n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list