BT-B - Discontinued

human (Homo sapiens)

cervix carcinoma (cell line HELA)

Caution: Cytogenetic and STR analyses established unequivocally that BT-B is genetically identical to HELA. BT-B must not be used as a model for urinary bladder carcinoma.

1

The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.

A

epitheloid cells growing adherently in monolayers

90% RPMI 1640 + 10% h.i. FBS

split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA

at 37 °C with 5% CO₂

about 43 hours

cell harvest of about 0.2-0.3 x 10⁶ cells/cm²

frozen with 70% medium, 20% FBS, 10% DMSO

initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis resulted in the STR profile of cell line HELA.

human hypertriploid karyotype with 13% polyploidy - 72(68-74)<3n>XX, -Y, +1, -4, +5, +5, +5, -11, +12, +12, -19, -21, -22, +3mar, der(1)t(1;3)(p11;q11), der(1)t(1;5) (p21;q21), der(2)t(2;5)(p11;q11), del(3)(q13), add(4)(q35), del(5)(p12), i(5p)x3, add(6)(q25/27), i(7q), del(8)(q23), del(10)(p11), inv(12)(q21q24)/der(12)t(12;?)(q21;?)x2, add(19)(p13) - mar1 resembles i(Xp) - HELA markers present

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV +, SMRV -

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