SBC-7 - Discontinued
ACC 229
Cell line:
SBC-7 - Discontinued
DSMZ no.:
ACC 229
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma (cell line HELA)
Origin:
Caution: Cytogenetic and STR analyses demonstrated that cell line SBC-7 is genetically identical to HELA. SBC-7 cells must not be used to model urinary bladder carcinoma.
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent epitheloid cells growing in monolayers
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
split confluent culture 1:5 to 1:10 about twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 15-17 hours
Harvest:
cell harvest of about 1-3 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis resulted in the STR profile of cell line HELA.
Cytogenetics:
human hypertriploid karyotype with 15% polyploidy - 73/74(70-77)<3n>XX/XXX, +1, -4, +5, +6, -9, -9, +11, -13, -15, +16, +16, +17, +17, -22, -22, +4mar, der(1)t(1;14)(p11;q11), i(3q), del(3)(q11), add(5)(p11), i(7q), der(8)t(8;?11)(q24;q11), i(11p)x2, add(15)(p11), del(17)(p12)x2 - HELA markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -
Supplied as: