PB-1
ACC 241
Cell line:
PB-1
DSMZ no.:
ACC 241
Species:
murine (mouse) (Mus musculus)
Cell type:
pre B cells
Origin:
established from the bone marrow of a CBA/C57BL mouse in 1995; cells are constitutively IL-7 dependent
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
85% McCoy's 5A + 15% non-heat inactivated FBS + 50 ng/ml human IL-7 + 0.4x MEM vitamins + 1x MEM non-essential amino acids + 0.5x essential amino acids + 1 mM sodium pyruvate + 0.12% sodium bicarbonate + 2 mM L-glutamine + 50 µM mercaptoethanol
Subculture:
cells are difficult to culture! split saturated culture 1:2 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells may initially grow rather slowly for about one week; maintain at 1-3 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
maximum density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
IL-7 dependency confirmed by 3H-thymidine assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list