COLO-679
ACC 264
Cell line:
COLO-679
DSMZ no.:
ACC 264
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established in 1985 from the soft tissue metastasis of a malignant melanoma from a 47-year-old woman
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
split confluent culture 1:5 to 1:10 using trypsin/EDTA; change medium twice weekly; seed out at ca. 2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 34 hours
Harvest:
cell harvest of about 25 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human, flat-moded, hypotetraploid karyotype with 8% polyploidy - 83-92<4n>XXXX, -1, +3, -4, +7, +7, -14, -15, -15, -16, -18, -20, -20, +3mar - del(3)(p11)x1-2, del(3)(q13.3), add(7)(q35)x2, del(9)(p22)x2, add(10)(p11)x1-2, add(10)(p13), add(14)(p11), add(19)(p13.3)x2, add(21)(p11), add(22)(q13)x4 - extensive subclonal variation of chs 1, 5 and 7 - 9p- recurrent in melanoma
Molec. Genetics:
genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list