S-117
ACC 266
Cell line:
S-117
DSMZ no.:
ACC 266
Species:
human (Homo sapiens)
Cell type:
thyroid sarcoma
Origin:
established from the sarcoma of the thyroid gland from a 47-year-old woman in 1985
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:2 about once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 37 hours
Harvest:
cell harvest of about 0.5-1.0 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hexaploid/octaploid karyotype with 14% polyploidy - 156(140-188)<6n>XXXX, -X, -X - multiple copies of: i(1p), i(1q), i(3p), i(6p), i(9q), add(5)(p15), add(16)(q23), i(mar) - 1-3 dmin/rings in 40% cells - isochromosome formation previously reported in thyroid tumors
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list