BM-1604 - Discontinued
ACC 298
Cell line:
BM-1604 - Discontinued
DSMZ no.:
ACC 298
Species:
human (Homo sapiens)
Cell type:
prostate carcinoma (cell line DU-145)
Origin:
Caution: Cytogenetic and STR analyses documented that BM-1604 is genetically identical to human prostate carcinoma cell line DU-145.
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing as monolayers
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
split confluent culture using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
doubling time highly variable, about 60 hours
Harvest:
cell harvest of about 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis resulted in STR profile of cell line DU-145.
Cytogenetics:
human hypotriploid karyotype with 22% polyploidy - 59(55-63)<3n>X, -X/Y, -Y, -2, -3, -4, -7, -8, -14, -16, -19, -20, -21, -22, +5mar (inc bisat x2-3), del(1)(p31), del(2)(p12), i(5p), del(6)(q22)/add(6)(q?12), der(6)t(4;6)(q22;p22.3), del(9)(p21), add(10)(q24-q25), add(11)(p11), t(11;?22)(q24;q13)/del(11)(q23), der(12)t(5;12)(q13;p12.3), add(13)(q33)x2, add(13)(q33), add(15)(p11), der(18)t(14;18) (q11;q12.3)x1-2 - sideline with del(2)(p23), i(8q) - closely related to DU-145
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as: