DV-90
ACC 307
Cell line:
DV-90
DSMZ no.:
ACC 307
Species:
human (Homo sapiens)
Cell type:
lung adenocarcinoma
Origin:
established from the pleural effusion of a 50-year-old man with lung adenocarcinoma (stage IV) in 1990; cells were described in the literature to be tumorigenic in nude mice; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1x non-essential amino acids
Subculture:
seed out ca. 2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA; after seeding, cells may need 2-3 days to adhere
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 95 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 17% polyploidy - 46(41-47)<2n>X, -Y, +8, add(6)(q27) - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list