HPD-1NR

Syrian hamster (golden hamster) (Mesocricetus auratus)

pancreas ductal adenocarcinoma

established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster (Mesocricetus auratus) induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-2NR (DSM ACC 293); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene

1

A

adherent epithelial cells growing mostly in monolayers (about 2-5% very large cells), but also with formation of a three-dimensional grid-like structure with some round, only loosly bound cells; image; image

80-90% RPMI 1640 + 10-20% h.i. FBS

split confluent culture before reaching confluence 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at about 2 x 10⁶ cells/80 cm²; cells have a high metabolic activity acidifying the culture medium; exchange spent medium every 2 days

at 37 °C with 5% CO₂

ca. 60-70 hours

cell harvest of about 6-7 x 10⁶ cells/80 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization assays

Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species.

near triploid highly rearranged karyotype with 36% polyploidy - 60-66<3n>XX, -X - hamster markers present - resembles published karyotype

ELISA: reverse transcriptase positive; PCR: SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

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