Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the left maxilla of a 12-year-old boy with Burkitt lymphoma in 1963; first continuous human hematopoietic cell line; cells carry a t(8;14) translocation leading to MYC-IGH fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) and in contrast to other EBV infected cell lines, the cells can be handled under biosafety level 1 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain between 0.25-1.0 x 106 cells/ml; split saturated culture 1:4 to 1:8 twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24-36 hours
Harvest:
maximum density at ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylamda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with 12% polyploidy - 89(80-91)<4n>XXYY, -4, -9, -12, -16, -18, -21, +3mar, add(4)(q35), add(6)(q27)x2, t(8;14)(q24;q32), der(8)t(8;14) (q24;q32)add(8)(?p12), del(9)(p23), der(14)t(8;14)(q24;q32) - carries t(8;14) associated with Burkitt lymphoma - closely resembles depositor's karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
