SK-MEL-3
ACC 321
Cell line:
SK-MEL-3
DSMZ no.:
ACC 321
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the lymph node metastasis of a malignant melanoma from a 42-year-old woman
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2;
split confuent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA;
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-tetraploid karyotype with 3% polyploidy - 92(80-93)<4n>XX, -X, -X, -6, +7, +7, +7, -10, -10, -16, -16, +20, +20, +20, +20, +22, dup(7)(p15p22)x2, der(9)t(7;9)(p11;p21)dup(7)(p15p22)x2 - sideline with add(2)(p23) marker - resembles published karyotype
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list