RLC-18
ACC 332
Cell line:
RLC-18
DSMZ no.:
ACC 332
Species:
rat (Rattus norvegicus)
Cell type:
embryonal liver
Origin:
established from the normal liver of a 17-day rat embryo (strain JAR-2) in 1975
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:4 every 3 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 + 7-10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 hours
Harvest:
harvest of ca. 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat hypertriploid karyotype with 13% polyploidy - 64(58-66)<3n> - large submetacentric marker present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list