DEL

human (Homo sapiens)

malignant histiocytosis

established from the pleural effusion of a 12-year-old boy with malignant histiocytosis at diagnosis in 1987 (problably anaplastic large cell lymphoma, ALCL); initially established by serial passage in nude mice; cells carry the NPM1-ALK (NPM-ALK) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

14370, 14485, 14492, 14464, 14737, 15185, 18187

1

A

grows often in clusters in suspension, most cells are polygonal (some cells are round); image; image

80-90% RPMI 1640 + 10-20% h.i. FBS

seed out at ca. 0.4 x 10⁶ cells/ml; split saturated culture 1:3 to 1:5 every 2-3 days

at 37 °C with 5% CO₂

ca. 30 hours

maximal density at ca. 1.5 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

CD3 -, CD5 -, CD6 -, CD7 -, CD8 -, CD10 -, CD14 -, CD19 -, CD25 +, CD30 +, CD34 -; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human biclonal, hyperdiploid/hypertriploid, karyotype with 6.7% polyploidy - 47(44-49)<2n>XY, +5, +5, +6, -8, +mar, der(5)t(5;6)(q35;p21), t(5;6)(q35;p21), add(10)(q23-24), der(13)t(1;13)(q32;p11)t(1;13)(q21;q34), add(16)(q23), add(19)(p13), 33% - 74(74-77)<3n>XXY, +1, +3, +5, +5, +6, +7, -10, +13, -18, +20, t(5;6)(q35;p21)x2, add(10)(q24)x1-2, der(13)t(1;13)(q32;p11)t(1;13)(q21;q34)x2, add(16)(q23), add(19)(p13), 60% - matches published karyotype, t(2;5) not detectible cytogenetically

expression of NPM1-ALK (NPM-ALK) fusion gene was detected by RT-PCR analysis

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

Print data sheet