MHH-PREB-1
ACC 354
Cell line:
MHH-PREB-1
DSMZ no.:
ACC 354
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the lymph node of a 5-year-old boy with B cell lymphoblastic non-Hodgkin lymphoma at diagnosis in 1994
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90-92.5% RPMI 1640 + 7.5-10% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 105 cells/ml; maintain at 0.5-2.0 x 106 cells/ml; split 1:10 to 1:20 twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-40 hours
Harvest:
cell harvest of ca. 2.5 x 106/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 12% polyploidy - 48<2n>XYY, +21, ?ins(2;2)(q21;p15p22), t(8;14)(q24;q32)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list