KYSE-180
ACC 379
Cell line:
KYSE-180
DSMZ no.:
ACC 379
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
established from the well differentiated squamous cell carcinoma resected from middle intra-thoracic esophagus of a 53-year-old man prior to treatment (tumor was invading contiguous structures)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at ca. 2-3 x 106 cells/80 cm2 in 10-15 ml; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 30 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 11% polyploidy - 55(50-57)<2n>XX, +1, +1, +3, +7, +7, +8, -14, +16, -17, -17, -18, -18, +5-8mar, del(X)(q23), add(1)(q32), der(1;6)(q10;p10), der(1;13)(q10;q10), add(3)(p26), add(5)(q25), add(6)(p11), del(6)(q23), add(7)(p11), del(7)(p11), add(7)(q35), i(7q), del(8)(p22), add(10)(p11), add(11)(q11), del(11)(q11q14), add(13)(p11), add(13)(q33), add(14)(p11), add(15)(p11), add(21)(p11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list