LCLC-97TM1

human (Homo sapiens)

large cell lung carcinoma

cell line represents the xenotransplant of a primary tumor which was obtained from a 44-year-old man with large cell lung carcinoma who did not have any prior therapy; listed in the CCLE (ref 18232)

14382, 18232

1

A

adherent epitheloid cells, densely packed in islets, growing as monolayers; image; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 2 x 10⁶ cells/80 cm²; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA before reaching complete confluency; medium (culture supernatant) should be exchanged at least every second day (even with splitting the cells)

at 37 °C with 5% CO₂

>50 hours

cell harvest of ca. 10 x 10⁶ cells/80 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hyperdiploid/hypotriploid karyotype - 58(50-60)<3n>X, -X/Y, -X/Y, +1, +1, +5, +5, -7, -10, -11, +12, -13, -15, -15, -15, -17, -18, -18, -19, -20, -21, -21, -22, der(1;7)(q10;q10)x2, der(1;15)(p10;q10)x2, der(3;6)(q10;q10), der(5;18)(p10;p10), der(5;18)(q10;q10), der(6;10)(p10;q10), i(7p)x2, i(8p), i(8q)x2, der(11)t(1;11)(q11;q24), der(14)t(7;14)(q12;q32), der(14)t(7;14)(q12;q32)add(14)(p11), der(14)t(7;14)(q12;q32)t(14;21)(p10;q10) - carries an unprecedented cluster of whole-arm translocations - resembles published karyotype

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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