RMB-1
ACC 391
Cell line:
RMB-1
DSMZ no.:
ACC 391
Species:
murine (mouse) (Mus musculus)
Cell type:
myeloid leukemia
Origin:
tumor induced in nude mouse injected with blast cells from ascites fluid of newborn Balb/c mouse injected intraperitoneally with Rauscher murine leukemia virus (supernate of producer cell line JLS-V9); descibed to be heterotransplantable, to form colonies in soft agar, to display myeloid cytochemical (myeloperoxidase) and functional (phagocytosis) features, and to differentiate terminally in response to DMSO
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
maintain at 1-2 x 106 cells/ml; split saturated culture 1:4 to 1:10 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-35 hours
Harvest:
maximal density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hyperdiploid karyotype - 41(38-41)<2n>XY, structurally and numerically rearranged
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list