BD-215

ACC 398

Cell line:

BD-215

DSMZ no.:

ACC 398

Species:

human (Homo sapiens)

Cell type:

B lymphoblastoid cells (from patient with Fanconi anemia)

Origin:

B lymphoblastoid cell line (B-LCL) established from the peripheral blood of a man with Fanconi anemia (complementation group C); homozygote with unrelated non-consanguineous parents; homozygous for nonsense mutation in exon 6 of the FANCC (FACC) coding sequences

Reference(s):

14559

Biosafety level:

Risk assessment:

The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.

Permissions and restrictions:

A, B

DSMZ Cell Culture Data:

Morphology:

lymphoblastoid-like cells growing as single cells or in clumps in suspension; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

split saturated culture 1:2 to 1:5 every 2-4 days; maintain at 0.4-1.0 x 10⁶ cells/ml; seed out at ca. 0.8 x 10⁶ cells/ml; after recovery from the frozen stock, first culture with 20% FBS

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 40-50 hours

Harvest:

saturation density at ca. 2.0-2.5 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 +, HLA-DR +, sm/cyIgM -, smIgG -, cyIgG +, sm/cykappa -, sm/cylambda -

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human diploid karyotype with 12% polyploidy - 46, XY, 1qh+ - no consistent abnormality detected

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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