TANOUE
ACC 399
Cell line:
TANOUE
DSMZ no.:
ACC 399
Species:
human (Homo sapiens)
Cell type:
B cell leukemia
Origin:
established in 1990 from the peripheral blood of an 11-year-old boy with acute lymphoblastic B-cell leukemia (ALL FAB L2)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 1-2 x 106 cells/ml; split ratio of 1:3 to 1:4 once a week; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
maximal density at ca. 3-5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 (+), CD38 +, cyCD79a +, CD138 +, HLA-DR (+); image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 12% polyploidy - 47/48<2n>X, -Y/XYqh+, +7, +14, dup(1)(q21.1/21.2q23.1/23.2), t(2;4)(q2?2;q2?5), del(6)(q27), t(8;14)(q24;q32), del(13)(q34), add(22)(q13) - extensive subclonal variation - carries t(8;14), +7, and dup(1q) typical of B-ALL/Burkitt lymphoma - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list