GMS-10
ACC 405
Cell line:
GMS-10
DSMZ no.:
ACC 405
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
established from the malignant brain tumor of a 49-year-old man with glioblastoma in 1995
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% DMEM + 10-15% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/80 cm2 ; split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50-70 hours
Harvest:
cell harvest of ca. 5 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 11% polyploidy - 69-76<3n>XXYY, +Y, +2, +3, +9, -10, -12, +15, +15, -18, +20, +20, -22, +2-4mar, +1-4dmin, del(2)(p11), add(2)(q31;hsr), der(4)t(3;4)(p21;p15.3), add(4)(p1?), del(6)(q23), add(7)(q11), add(9)(p23), add(13)(p11), add(15)(p11)x3, add(19)(q13), add(21)(p11)x3 - same hsr present in mar and add(2)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list