U-2197
ACC 406
Cell line:
U-2197
DSMZ no.:
ACC 406
Species:
human (Homo sapiens)
Cell type:
malignant fibrous histiocytoma
Origin:
established from the subcutaneous malignant fibrous histiocytoma of a 79-year-old man in 1984 (third recurrence after repeated surgery, radially invading the thumb extensor); described in the literature to express high levels of PDGF-A which is not secreted
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% MEM (with Earle's salts) + 20% h.i. FBS + 0.13% Na-bicarbonate
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; maintain at 6 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100-120 hours
Harvest:
cell harvest of ca. 8 x 106 cells/175 cm2; maximal density of 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertetraploid karyotype with hypotriploid sideline and 20% polyploidy - 100(92-101)<4n>XXXX, -2, -4, +6, +6, +7, -8, -9, -9, +14, +14, -15, -15, -16, -16, +19, -20, -20, -21, -21, +13mar, i(Xq)x2, add(X)(p11)x2, del(1)(q11)x2, del(4)(p11), add(6)(q15)x2, i(6p)x2-4, add(7)(q35)x2, add(7)(p14)x2, del(7)(p11), add(12)(q24)x2, add(13)(p11)x2, add(13)(p11)x2, add(19)(q13), del(19)(q13)x2 - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list