SER-W3
ACC 420
Cell line:
SER-W3
DSMZ no.:
ACC 420
Species:
rat (Rattus norvegicus)
Cell type:
Sertoli cells
Origin:
Sertoli cells dissected from normal testis from 17-day-old Wistar rat and immortalized with SV40 large-T antigen; cells were described as displaying morphological and functional features of Sertoli cells (phagocytosis, cisplatin sensitivity, transferrin secretion)
Reference(s):
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
split 90% confluent culture 1:2 to 1:5 every 2-6 days using trypsin/EDTA; seed out at ca. 0.3-0.5 x 105 cells/cm2; maintain at ca. 0.1-0.3 x 105 cells/cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 0.8 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat flat-moded hypotetraploid karyotype - 70-78<4n>XXYY/XXY - supernumerary r/acf present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list