SW-1710
ACC 426
Cell line:
SW-1710
DSMZ no.:
ACC 426
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell cacrinoma
Origin:
established from the bladder tumor of an 84-year-old woman following transurethral tumor resection in 1977
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% DMEM + 15% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:3 to 1:5 every 2-4 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 25-32 hours
Harvest:
cell harvest of ca. 6-8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with 8% polyploidy - 77-84<4n>X, -X, -X, -X, -4, -4, -5, -5, +6, +6, -8, -9, -10, -11, -13, -14, -14, -16, -19, +20, +20, +4mar, der(X)t(X;6)(q11-13;q25-q27), del(2)(p12)x2, add(3)(p14-21), der(6)t(X;6)(q11-13;q25-27)x2, der(6)del(6)(p11p12.2)add(6)(q25-27)x2, add(13)(q33-34) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list