Leibniz Institute DSMZ: Details

Cell line:

42-MG-BA

DSMZ no.:

ACC 431

Species:

human (Homo sapiens)

Cell type:

glioblastoma

Origin:

established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment)

Reference(s):

14846

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes; image; image; image

Medium:

90% mixture of RPMI 1640 + MEM (with Earle's salts) (at 1:1) + 10% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/80 cm²; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; saturation density at ca. 0.6 x 10⁵/cm²

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 40 hours

Harvest:

cell harvest of maximal 0.3-0.5 x 10⁵/cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)add(2)(p2?4), del(3)(q13.3), add(4)(p11), add(5)(p1?5), add(6)(q2?)x2, add(7)(q11)x2, add(7)(q3?), del(9)(p21p22)x2, del(11)(p11)x2, inv(12)(q12q21)x2, der(12)del(p11)t(7;12)(q21;q1?4)x2, add(13)(p11)x2, add(14)(q32)x2, add(21)(p11)x2

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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