JAR

human (Homo sapiens)

choriocarcinoma

established from the trophoblastic tumor of the placenta of a 24-year-old woman, but the cell line is from the male fetus

1

A

epithelial-like cells growing adherent in monolayers and in clusters; image; image; image

85% RPMI 1640 + 15% h.i. FBS

split culture 1:5 to 1:10 every 3-4 days before reaching confluency using trypsin/EDTA; seed out at ca. 3-4 x 10⁶ cells/80 cm²; confluent cultures tend to form aggregates, that loosely adhere to the culture vessel surface; however, these aggregates are difficult to disperse and may need trypsin treatment after a centrifugation and washing step

at 37 °C with 5% CO₂

about 20-30 hours

cell harvest of ca. 10-15 x 10⁶ cells/80 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human flat-moded near-triploid karyotype with 27% polyploidy - 60-70<3n>XXY, +1, -2, -5, -7, -8, -9, -13, -13, -14, -15, -17, -19, -21,+12mar, +acf, add(X)(p22), del(1)(p3?p3?), add(1)(q11), der(1)add(1)(p11)add(1)(q4?), inv(3)(p25.2q24), add(3)(q13), add(4)(q?31), del(4)(q2?2), add(9)(p21), add(12)(q24), i(13)(q10)dup(13)(q11q34), add(16)(q24), add(17)(p12), add(18)(p11.2) - sideline with add(2)(p2?), del(8)(q22), add(21)(p11) - resembles published karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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