TALL-1
ACC 521
Cell line:
TALL-1
DSMZ no.:
ACC 521
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the bone marrow of a 28-year-old man who developed the terminal leukemic phase of lymphosarcoma in 1976
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-4 days; maximal density of ca. 2.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
cell harvest of about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays
Immunology:
CD2 +, cyCD3 +, CD4 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype - 90-102<4n>XXY/XXYY, -3, -9, -12, +13, +14, +14, -15, +20, +20, +21, +21, +2-4mar, der(X;1)(p10;p10)/add(1)(q11), add(1)(p11), add(5)(p1?5), del(2)(p22p24), del(12)(q24.1), dup(21)(q11qter)x3-4 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list