Species:
human (Homo sapiens)
Cell type:
T cell lymphoma
Origin:
established from the bone marrow blood of a 30-year-old man with hepatosplenic gamma-delta T cell lymphoma (T-cell NHL) in 1995 at disease progression; cells are IL-2 dependent; simultaneous sister cell line of cell line DERL-2 (ACC 531). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
small ovoid ("lymphoblastic") cells growing singly or in small clumps in suspension;
image;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml human IL-2
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml in 12-well-plates for optimal growth, cultures may be continued in flasks after 2-3 weeks following recovery; split saturated culture 1:2 to 1:3 every 2-4 days; cultures may contain significant amounts of cell debris; maximal density of ca. 3.0 x 106 cells/ml; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and PCR assays
Immunology:
CD2 +, CD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, HLA-DR -, TCRalpha/beta -, TCRgamma/delta -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 47(46-47)<2n>XY, +8, i(7q), add(10)(p12.1) - sideline with der(1)t(1;16)(q12.2;p13.3) - i(7q) is associated with hepatosplenic T-NHL - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
