Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 40-year-old man with acute myeloid leukemia (AML) FAB M4Eo at second relapse in 1988; cells were described in the literature to carry the inv(16)(p13q22) leading to the fusion gene CBFB-MYH11. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round cells growing singly in suspension;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml in 24-well-plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 3-5 days; minimum density at ca. 0.5 x 106 cells/ml; we recommend to cultivate cells allways in 12- or 24-well-plates
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-5 days
Harvest:
maximum cell density of ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD4+, CD13+, CD14-, CD15-, CD19-,
CD33+, CD34+, cyCD68-;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 9% polyploidy; 48(45-48)<2n>XY, +8, +20, der(2)t(2;11)(q31;q13), der(9)t(9;18)(q12.2;q11), der(16)t(11;16)(q13;q22)inv(16)(p13q22), der(17)t(2;17)(p25;p12); sdl lacking trisomy 20; carries inversion/translocation effecting rearrangement of MYH11 and CBFB; resembles published karyotypes
Molec. Genetics:
expression of fusion gene CBFB-MYH11 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
