H-2171
ACC 544
Cell line:
H-2171
DSMZ no.:
ACC 544
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
derived from the pleural effusion of a 50-year-old white male with extensive small cell lung carcinoma after chemotherapy in 1989; corresponds to NCI-H2171; listed in the CCLE (ref 18232) with extensive genome and protein analyses
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:2 to 1:3 every 2-4 days; seed out at ca. 1.0 x 106 cells/ml; maintain at 0.7-2.0 x 106 cells/ml; after recovery the growing is slow
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-60 hours
Harvest:
maximum density at 3.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 8% polyploidy - 42-44<2n>XY, -4, -5, -8, -10, -11, -13, -15, -17, -19, +20, +4-5mar, add(1)(p36), der(2)ins(2;?)(p2?;?)t(2;2)(q37;p24), add(3)(p11), i(3q), del(6)(q25q26), del(9)(p11), der(12)add(12)(p11)del(12)(q24), add(14)(p11), i(15q), add(18)(p11)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list