NU-DUL-1
ACC 579
Cell line:
NU-DUL-1
DSMZ no.:
ACC 579
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1982 from the cerebrospinal fluid of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as undifferentiated lymphoma; assigned to ABC-like lymphoma subtype (activated B-cell)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
polymorphic cells growing singly in suspension; image
Medium:
85-90% RPMI 1640 + 10-15% h.i. FBS
Subculture:
seed out at ca. 0.3 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3 days; maintain at ca. 0.5-0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-36 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 4% polyploidy - 45<2n>X, -Y, der(X)t(X;13)(p11.2;q13), del(3)(q26-28), der(5)t(X;5)(q26;q21), del(6)(p21p22), del(6)(q22), der(8)t(8;14)(q24;q32), add(11)(q23-24), der(14)t(8;14)(q24;q32)t(14;13)(q32;?q14)t(13;X)(?q13;p11.2), der(17)t(17;18)(q22;q21.1), der(18)t(5;18)(q21;q21.1)inv(5)(q21;q35), der(19)t(?17;19)(q22;q13.2) - carries semi-cryptic t(8;14) effecting IGH-MYC rearrangement - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list