WSU-NHL
ACC 58
Cell line:
WSU-NHL
DSMZ no.:
ACC 58
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the pleural effusion of a 48-year-old woman with nodular histiocytic lymphoma (refractory, progressive) in 1986; reported to carry a t(14;18) leading to the IGH-BCL2 fusion gene and to express mRNA for the BCL2 oncogene; assigned to GCB-like lymphoma subtype (germinal center B-cell)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-0.8 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells; split 1:2 to 1:4 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 45 hours
Harvest:
maximum density at about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cykappa -, sm/cylambda +, sm/cyIgG +, sm/cyIgM -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 6% polyploidy - 45(42-45)<2n>XX, -4, -15, +mar, der(1)t(1;4)(p36;q11), der(1)t(1;?7)(p34;q31.2)t(1;16)(q22;q22), del(5)(q32q35), der(6)t(1;6)(q22;p21), t(9;13)(p22;q13-14), der(13)t(13;?16)(p11;p11), t(14;18)(q32;q21), der(16)t(16;?)(q22;?)t(?;5)(?;q31), add(17)(p1?), add(19)(q13.2) - matches published karyotype - carries t(14;18) associated with NHL
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list