Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the left inguinal lymph node of a 73-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma, non-cleaved cell type) in 1982; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
single, round to polymorph cells growing in suspension;
image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out and maintain at ca. 0.5-1.0 x 106 cells/ml; it is recommended to start culture in a 24-well-plate and with 20% FBS; split saturated culture 1:2 to 1:4 every 2-3 days; viability of culture may drop to about 50% after thawing and may need more than one week to recover
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours
Harvest:
maximum density at about 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, fm/cykappa -, sm/cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 51(46-53)<2n>XY, +5, +8, +9, +12, +12, t(3;8)(p25;q24), i(5p), dup(7)(q21.3q31.1), der(8)t(3;8)(p25;q24), i(9p), del(12)(q11), i(12p), der(14)t(14;18)(q32;q21)x1-3 - sideline with dup(2)(p2?1p2?4) - carries t(3;8) and t(14;18) effecting respective rearrangements of MYC and IGH-BCL2 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
