HNT-34
ACC 600
Cell line:
HNT-34
DSMZ no.:
ACC 600
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1994 from the peripheral blood of a 47-year-old woman with acute myeloid leukemia (AML FAB M4) secondary to previous myelodysplastic syndromes (MDS), specifically chronic myelomonocytic leukemia (CMML); described in the literature to carry the t(3;3)(q21;q26) leading to MECOM (EVI1) overexpression and the Philadelphia chromosome t(9;22) expressing both P190 and P210 BCR-ABL1 chimeric transcripts
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.8 x 106 cells/ml initially in a 24-well-plate; viability may drop below 50% during the first few days and a significant amount of cell debris is always visible; in logarithmic growth phase split culture 1:2 to 1:3 every 2-3 days and maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density at about 3 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 2-3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-diploid karyotype with 7% polyploidy; 46(44-48)<2n>XX, t(3;3)(q21;q26), t(9;22)(q34;q11), del(17)( ?p12.2p13.1), del(20)(q13.1q13.2); t(3;3) and t(9;22) effect rearrangement of MECOM (EVI1) and BCR-ABL1, respectively; matches published karyotype.
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list