L-591
ACC 602
Cell line:
L-591
DSMZ no.:
ACC 602
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells (from patient with Hodgkin lymphoma)
Origin:
established from the pleural effusion of a 31-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB) in 1982
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split culture 1:2 twice a week; seed out at ca. 0.5 x 106 cells/ml in 24-well-plates; optimal growth is achieved using 24-well-plates, but culture can be adapted to grow in flasks with lower viabilities; maintain at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 72 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 +, CD3 -, CD4 -, CD8 -, CD10 -, CD19 +, CD20 -, CD21 +, CD25 +, CD30 +, CD34 -, CD80 +, CD138 -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 6% polyploidy; 46(45-48)<2n>XX, add(7)(q32), der(14)t(9;14)(p13;q32); der(14) effects rearrangement of PAX5 which (untypically for HL) is upregulated
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list