Leibniz Institute DSMZ: Details

Cell line:

U-HO1

DSMZ no.:

ACC 626

Species:

human (Homo sapiens)

Cell type:

Hodgkin lymphoma

Origin:

established in 2005 from the pleural effusion of a 23-year-old man with terminal refractory classical Hodgkin lymphoma (nodular sclerosing type) after radiotherapy, salvage chemotherapy and autologous stem cell transplantation; described in the literature to carry an amplification of REL-BCL11A region with overexpression of miR-216/217

Reference(s):

14728, 40211

Biosafety level:

1

Permissions and restrictions:

A

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

polymorphic cells growing singly and in clumps in suspension; occasionally large cells present; image; image; image; image

Medium:

80% Iscove's MDM + RPMI 1640 (at 4:1) + 20% h.i. FBS, 2 mM L-glutamine

Subculture:

seed out at ca. 1-1.5 x 10⁶ cells/well in a 24-well-plate; optimal split ratio 1:2 every 3-5 days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 100 hours

Harvest:

maximum density at ca. 2 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 +, CD34 -, HLA-DR +; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hyperdiploid karyotype with 9% polyploidy; 51(46-52)<2n>XY, +5, +6, +8, +12, +17, +20, -22, +mar, del(1)(p13p31), der(2)qdp(2)(p1?5p1?6)t(2;10)(p24;q25.2), add(4)(q35), t(4;6)(p15.2;p21), del(5)(q11q34), der(5)t(5;15)(p11;q13), t(5;22)(q35;q13), del(6)(q13), der(10)t(2;10)(p24;q25.2)x1-2, t(12;18)(p10;q10), del(15)(q11q14), add(15)(q14), del(20)(q13)x1-2, dup(21)(q22.1q22.2), der(21)t(15;21)(q15;p11); genomic amplification of BCL11A-REL region on der(2); resembles published karyotype.

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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