CRO-AP6
ACC 650
Cell line:
CRO-AP6
DSMZ no.:
ACC 650
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established at diagnosis from the pleural effusion of a 26-year-old man with primary effusion lymphoma (PEL) in 1999; described in the literature to be HHV-8 +, EBV - and to display biallelic inactivation of the DNA repair enzyme gene O6-methylguanine-DNA methyltransferase (MGMT) by promoter methylation
Reference(s):
Biosafety level:
2
Risk assessment:
The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
round to pleomorphic cells growing in small clumps in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in a 24-well-plate; optimal split ratio of 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.0 x 106 cells/ml; after thawing, viability may drop to 50-60%, but cells recover after 4-5 days; during the first days of culture change medium only
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum densitiy of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 48(45-48)<2n>XY, +16, +mar, der(11)t(4;11)(q28;q24), der(13)dup(13)(q1?q3?)t(13;14)(q3?;q21), der(14)t(13;14)(q3?;q21), add(16)(q24)(hsr), i(21)(q10); carries hsr on der(13) and der(16); matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list