WILL-2
ACC 652
Cell line:
WILL-2
DSMZ no.:
ACC 652
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 2008 post-mortem from the ascites of a 63-year-old woman with diffuse large B-cell lymphoma after relapse
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in a 24-well-plate; cells may grow slowly during the first days after thawing, but recover soon and grow fast in log-phase; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at 1-2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36-48 hours
Harvest:
maximum densitiy of ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD38 +, CD138 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype; 46(43-47)<2n>XX, del(X)(q23), dup(1)(q21q32), ins(1;X)(q21;q27q23), t(4;17)(p15;q12). t(5;9)(q14;p21), t(8;22)(q24;q11), t(14;18)(q32;q21); carries t(8;22) rearrangement effecting IGL-MYC, and t(14;18) rearrangements effecting IGH-BCL2; resembles published karyotype
Molec. Genetics:
expression of fusion gene IGH-BCL2 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list