YNH-1
ACC 692
Cell line:
YNH-1
DSMZ no.:
ACC 692
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 46-year-old man with acute myeloid leukemia (AML M1) at diagnosis in 1994; cells were described in the literature to be constitutively cytokine-dependent and cytokine-responsive; cells carry the translocation t(16;21)(p11;q22) leading to FUS-ERG (TLS-ERG) fusion gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
60% RPMI 1640 + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35) or 10 ng/ml G-CSF
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1 x 106 cells/ml; split saturated culture 1:2 every 3-4 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -;image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy; -47(44-48)<2n>XY, +19, add(7)(q35), der(16)t(1;16)(q12;q12)t(16;21)(p11;q22), der(21)t(16;21)(p11;q22; carries complex t(16;21) effecting rearrangement of FUS (TLS) with ERG; matches published karyotype
Molec. Genetics:
expression of fusion gene FUS-ERG (TLS-ERG) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list