OE-33
ACC 706
Cell line:
OE-33
DSMZ no.:
ACC 706
Species:
human (Homo sapiens)
Cell type:
esophageal adenocarcinoma
Origin:
established from the primary tumor of a 73-year-old woman with Barrett-esophageal adenocarcinoma in 1993; cells are described in the literature to produce tumors in nude mice; cell line also known as JROECL33; genome sequence data in ref 40712 and 40877
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture up to 1:10 every 4-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype; 92-100<4n>XX, -X, -X, +1, -4, +5, +6, +7, +7, +7, +7, +8, +10, +10, -13, -13, -13, +16, +16, +16, -17, -18, -18, -19, +20, +20, +20, +20, -21, -22, -22, +6mar; add(1)(p13)x2, i(3)(q10)x1-2, del(5)(p14.2), add(6)(q22), add(7)(q34)x3, der(11)add(11)(q23)i(11)(q10), add(14)(p11)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list