MOLM-1
ACC 720
Cell line:
MOLM-1
DSMZ no.:
ACC 720
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia
Origin:
established from the bone marrow of a 41-year-old man with chronic myelogenous leukemia (CML) in blast crisis; cells are described in the literature to display megakaryocytic features and to be inducible to megakaryocytic differentiation; cells carry the Philadelphia chromosome with an e13-a2 (b2-a2) BCR-ABL1 fusion gene and an inv(3) leading to MECOM (EVI1) overexpression
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round cells growing singly in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml in 24-well plate; cells may grow slowly in the first week after thawing; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-triploid karyotype; 66-70<3n>X, -X/Y, -X/Y, +1, -7, +13, -16, -18, +22, del(5)(q11q34.2), inv(3)(q21q26)x2, der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2;
carries BCR-ABL1 and MECOM (EVI1) rearrangements; matches published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list