Species:
human (Homo sapiens)
Cell type:
natural killer cell leukemia
Origin:
established 1997 from the peripheral blood of a 45-year-old woman with aggressive natural killer cell (NK) leukemia; cells were described in the literature to display strong cytotoxic activity and to carry a point mutation in exon 7 of the TP53 (p53) gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polymorph cells growing singly and mainly in clumps in suspension; a certain amount of cell debris may be present all the time;
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Medium:
90% RPMI 1640 + 10% h.i. FBS+ 10 ng/ml IL-2
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.1-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; viability may be low after thawing, but cells recover during the following 2-3 days, culture should be started with 20% FBS in 24-well-plates; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours in log phase
Harvest:
maximum density at ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 +, cyCD3 +, smCD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human highly rearranged flat-moded hypertetraploid karyotype with 2.5% polyploidy; 84-96<4n>XXXX, +8, +8, +8, -9, t(5;6)(q21;q24)x2, der(7)t(4;7)(q31;q33)x2, der(7)dup(7)(q?31q?35)t(5;7)(p15;q35)x2, der(8)t(8;8)(p22;q24)inv(8)(q12q24)x2, der(9;10)(q10;q10), dic(10;20)(p12;q13), der(10)t(10;13)(p11;q11)t(13;16)(q21;q12)x1-2, der(11)t(7;11)(q22;q24)x2, der(15)t(15;16)(p13;q12)x2, der(16)t(13;16)(q21;q12)x2, i(18)(q10)x2, der(20)t(7;20)(q22;q13)x2; copy number increases affecting BCL2/18q21 and MYC/8q24 regions; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
